Stay updated with the latest research milestones, publications, and events from The Mayer Lab.
After characterizing the mammalian CROP1 complex involved in the fission of endosomal Retromer coated tubules, here is the CROP2 complex involved in the fission of Retriever coated tubules !!!
You thought that each Sorting Nexin / Retromer complex defined a specific recycling route ? Well you might have to change your view...
Ever heard about acidocalcisomes ? Well this almost ubiquitous organelle is worth reading about. Learn more with our paper "Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol" in eLife. In collaboration with Samuel Bru from the Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya, Barcelona, Spain
Utilizing pulse-labelling experiments with 18O water and ordinary differential equation (ODE) models, we explore the synthesis and turnover of the highly phosphorylated IPP, 1,5-InsP8. Our findings challenge the notion that 1,5-InsP8 can be synthesized through distinct routes, revealing a linear reaction sequence in both systems.
A fruitful collaboration with Jacques Hermes and Jens Timmer at the Institute of Physics, University of Freiburg, Freiburg, Germany.
We demonstrate a rapid (seconds to minutes) and nonradioactive labeling strategy of inositol polyphosphates through 18O-water in yeast, human cells, and amoeba, which can be applied in any media. In combination with capillary electrophoresis and mass spectrometry, 18O-water labeling simultaneously dissects the in vivo phosphate group dynamics of a broad spectrum of even rare inositol phosphates.
A collaboration with our friends and colleagues at the Institute of Organic Chemistry, University of Freiburg, Germany
A small contribution to a great study led by Kai-En Chen and Brett Collins at the University of Queensland.
Fluorescence recovery after photobleaching (FRAP) can be employed to investigate membrane lipid mixing of vacuoles in live budding yeast cells and distinguish the fused, hemi-fused or non-fused states of these organelles under physiological conditions. Here, we describe a protocol for labeling the outer and inner leaflets of vacuoles in live cells that allow detection of hemifusion intermediates and, thus, identify components necessary for fusion pore opening.
Assembly and fission of tubular carriers mediating protein sorting in endosomes
Read our new review written in collaboration with Kai-En Chen and Brett Collins in Nature Reviews Molecular Cell Biology